Establishment of OVS1 and OVS2 monoclonal antibodies recognizing human ovarian mucinous cystadenocarcinoma.

Two newly established murine monoclonal antibodies (MAbs), OVS1 and OVS2, to human ovarian mucinous cystadenocarcinoma were further characterized for diagnostic efficacy. The specific SA-1 antigen, purified from the tumor extract was identified as a glycoprotein of 29 kDa. A double determinant biotinstreptavidin alkaline phosphatase immunoassay system, containing OVS1 and OVS2 MAbs was used to determine the SA-1 levels in serum. The OVS1 MAb was used as a first antibody because of its high specificity of 96% while OVS2 MAb, with a lower specificity of 8% but greater sensitivity of 78%, was chosen as a second antibody. Matched sera of 64 healthy controls and 90 patients with definite diagnoses of 25 benign diseases, 14 nonovarian cancer and 51 ovarian cancer, were simultaneously measured together with CA 125 values. At cut-off levels of 220 and 360 units/ml, the SA-1 test showed 63% and 43% positive rates respectively in all types of ovarian cancer, compared to 65% and 57% positive rates for CA 125 at cut-off levels of 35 and 60 units/ml, respectively. Sensitivity for SA-1 at 220 units/ml cut-off level in mucinous ovarian cancer was 75% and increased significantly to 85% when the test was combined with CA 125 at 35 units/ml cut-off level. Furthermore, The combination of both tests significantly increased the positive rates to 86% in all types of early stage ovarian cancer.(ABSTRACT TRUNCATED AT 250 WORDS)

CSF finding in AIDS patients: Report of 112 cases.

One hundred and twelve cerebrospinal fluids from acquired immunodeficiency syndrome (AIDS) patients were analysed at Department of Neurology, Faculty of Medicine, Siriraj Hospital, Mahidol University during 1900-1993.The increasing rate rising from 3 (0.28%) cases to 12 (1.19%) cases, 33 (3.4%), 64 (7.4%) cases in 1990, 1991 1992 and 1993 respectively. The India ink preparation and latex agglutination revealed that 62 cases (-55%) of these patients had been infected with cryptococcus neoformans. Fach year the infection of this fungus in AIDS patients were increasing from 1 (33%) cases in 1990 to 5 (41%) cases in 1991, 20 (61%) case in 1992 and 36 (56%)cases in 1993. The mean value of white cell count, protein, and sugar of the AIDS patients infected with cryptococcus neoformans. were 39.43 cell/cumm (SD = 76.23), 77.91 mg/dl (SD = 62.91) and 35.46 mg/dl (SD = 19.48) respectively. These compositions were statistically significant lower than the AIDS patients that have the other complications. This study indicated that the AIDS patients have the low immune response to cryptococcus neoformans which is the major caused of the central nervous system disease in AIDS patients in Thailand.